Regulatory
AR A1-B1

Part:BBa_K2984025:Design

Designed by: Sophia Schiebler   Group: iGEM19_Humboldt_Berlin   (2019-10-15)


AR Promoter A1-B1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 282
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon syntax fits C. reinhardtii. This was designed as a A1-A3 part according to the MoClo standard of Patron et al. (2015).


Source

Schroda, M., et al. (2002). "Sequence elements within an HSP70 promoter counteract transcriptional transgene silencing in Chlamydomonas." Plant J 31(4): 445-455.

References